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ELISA Grade Collagen

Chondrex, Inc. provides type I and type II collagen specially purified for antibody assay by ELISA. Each vial of ELISA grade collagen contains enough collagen to coat ten 96-well plates. A 10X dilution buffer is also supplied as a coating buffer, which prevents fibril formation of collagen. Each vial of ELISA grade collagen contains 0.5 mg collagen in 1 ml of 0.05M acetic acid.

ELISA Grade Type I Collagen

Species Quantity Catalog # Price (USD)
Bovine 0.5 mg/ml x 1 ml 1002 87.00
Chick 0.5 mg/ml x 1 ml 1001 87.00
Human 0.5 mg/ml x 1 ml 1005 280.00
Mouse 0.5 mg/ml x 1 ml 1006 115.00
Porcine 0.5 mg/ml x 1 ml 1003 87.00
Rat 0.5 mg/ml x 1 ml 1004 115.00

ELISA Grade Type II Collagen

Species Quantity Catalog # Price (USD)
Bovine 0.5 mg/ml x 1 ml 2012 170.00
Chick 0.5 mg/ml x 1 ml 2011 170.00
Human 0.5 mg/ml x 1 ml 2015 340.00
Monkey 0.5 mg/ml x 1 ml 2017 215.00
Mouse 0.5 mg/ml x 1 ml 2016 480.00
Porcine 0.5 mg/ml x 1 ml 2013 170.00
Rat 0.5 mg/ml x 1 ml 2014 210.00

ELISA Grade Type III Collagen

Species Quantity Catalog # Price (USD)
Porcine 0.5 mg/ml x 1 ml 1093 115.00

Protocol for preparing ELISA grade collagen for coating plates

Diversity of Anti-Collagen Antibodies in CIA Models and Patients with Rheumatoid Arthritis

CIA-susceptible and even CIA-resistant animals generate very high levels of antibodies to the immunizing collagen. Importantly, antibodies in CIA-susceptible animals recognize the conserved epitopes shared by various species of type II collagen and cross-react to autologous type II collagen by 50-60%. Therefore, we recommend monitoring the antibody levels against the heterologous type II collagen used for immunization and an autologous type II collagen to correlate the development of arthritis and antibody levels.

Similarly, antibodies to type II collagen in patients with rheumatoid arthritis (RA) react dominantly with a heterologous type II collagen such as bovine or chick type II collagen, and cross-react to human type II collagen to a certain degree, depending upon individual patients.

Type I collagen from various species of animals are also available. Type I and type II collagen share 80% homology in their amino acid sequences, but their immunological features differ significantly as well as their tissue distribution.  These various species and types of collagen can be used to determine the antibody specificity in experimental animals and patients with a variety of autoimmune diseases.