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Collagen Induced Arthritis

Collagen

Chondrex, Inc. provides various species and types of highly purified type II, IX, and XI cartilage collagen for inducing Collagen-Induced Arthritis (CIA). CIA can be induced in rodents and non-human primates by immunization with type II collagen, but it has been reported that type XI is also capable of inducing arthritis in mice and rats, because the alpha 3(XI) chain of type XI collagen is identical to the alpha 1(II) chain of type II collagen. Furthermore, it has been suggested that autoantibodies to type IX collagen may contribute to polychondritis development in mice. Choose the appropriate species and types of collagen for individual experimental purposes.

For further information, see our Mouse CIA and Rat CIA Protocols.

Adjuvants

Collagen-Induced Arthritis (CIA) can be induced in certain strains of mice, rats, and non-human primates by immunization with type II collagen emulsified with adjuvant. To effectively induce CIA in animals, the type of adjuvant used for emulsifying collagen is a key element. Chondrex, Inc. provides both Incomplete Freund’s Adjuvant (IFA) and Complete Freund’s Adjuvant (CFA) containing various amounts of M. tuberculosis to optimize the immunization condition for inducing CIA.

**Please consult with your institution's animal care and use committee before ordering**

Note: For inducing CIA in rats, please use Incomplete Freund's Adjuvant for the emulsion preparation.  Rats are susceptible to adjuvant-induced arthritis (AIA) when immunized with CFA, which leads to the development of a histologically and immunologically distinct type of arthritis than the CIA model. 

Collagen - Type II

Species Quantity Catalog # Price (USD)
Bovine 10 mg, lyophilized 20021 195.00
Bovine 2 mg/ml x 5 ml 20022 195.00
Chick 10 mg, lyophilized 20011 195.00
Chick 2 mg/ml x 5 ml 20012 195.00
Porcine 10 mg, lyophilized 20031 195.00
Porcine 2 mg/ml x 5 ml 20032 195.00

Collagen - Type IX

Species Quantity Catalog # Price (USD)
Bovine 5 mg, lyophilized 1072 295.00
Chick 5 mg, lyophilized 1071 295.00
Porcine 5 mg, lyophilized 1073 295.00

Collagen - Type XI

Species Quantity Catalog # Price (USD)
Bovine 5 mg, lyophilized 1082 242.00
Chick 5 mg, lyophilized 1081 242.00
Porcine 5 mg, lyophilized 1083 242.00

Incomplete and Complete Freund's Adjuvant for CIA

Product Content of M. Tuberculosis H-37 RA Quantity Catalog # Price (USD)
Complete Freund's Adjuvant, 1 mg/ml 1 mg/ml 5 ml 7008 31.00
Complete Freund's Adjuvant, 2 mg/ml 2 mg/ml 5 ml 7009 36.00
Complete Freund's Adjuvant, 4 mg/ml 4 mg/ml 5 ml 7001 54.00
Complete Freund's Adjuvant, 5 mg/ml 5 mg/ml 5 ml 7023 73.00
Incomplete Freund's Adjuvant ----------- 5 ml 7002 28.00

Muramyl Dipeptide (MDP)

Product Catalog # Price (USD)
Muramyl Dipeptide, MDP, 2 mg 9065 74.00

The following information has to be considered for induction of arthritis.

Combination of MHC Types and Species of Collagen for Inducing CIA
Collagen-induced arthritis (CIA) can be induced in certain strains of mice, rats, and non-human primates by immunization with type II collagen emulsified with adjuvant. Susceptibility to CIA in rodents is linked to MHC class II molecules of animals, but also depends on the species of type II collagen used for immunization. Choose the appropriate species of type II collagen for inducing CIA according to the table below. Importantly, homologous type II collagen is not capable of inducing CIA by an ordinary immunization schedule.

Two Factors that should be considered by Users of Animal Models

Animal models have contributed to remarkable advances in the understanding of numerous human diseases.  As invaluable as animal models are, some drawbacks do exist.  One such drawback stems from the different sources of variability which can affect the outcome of the experiments.  Therefore, a pilot study is generally recommended for first time users of all animal models.  A brief discussion of the two main points of variability will be explained below.

1.    Animal Vendors

The genetic background of mice, even in the same strain, will vary from vendor to vendor and can affect how the animals respond to various reagents, thus impacting the results of your experiment (1).  Therefore, testing animals from different vendors using a defined protocol is recommended before proceeding with a full-scale experiment.

2.    Housing Conditions

The bacteria flora is very important to the health of the animal, including a properly functioning immune system (1).  Therefore, we recommend housing animals in Specific Pathogen Free (SPF) conditions rather than conventional conditions to avoid variability caused by bacteria.

Overall, special consideration of these main points of variability will aid the researcher in completing their experiments with less confounding results.

References

1.    Ivanov II. et al.  Induction of intestinal Th17 cells by segmented filamentous bacteria.  Cell 139(3): 485-498 (2009).