Monomeric and polymeric collagen in immunological assays
The quality of standards for assays is crucial for obtaining consistent results throughout long-term studies and between different studies. Using different batches of these reagents can impact assay validation and reproducibility. Chondrex, Inc.'s ELISA grade collagen undergoes filtration steps to remove polymeric collagen, ensuring a high ratio of monomeric collagen that is ideal for use as standards in sandwich ELISAs as well as antigens in indirect ELISAs.
To obtain absolute values of collagen levels in samples, monomeric collagen (ELISA grade collagen) must be used as a standard in ELISAs. Read More
Type III collagen purification and evaluation using SDS-PAGE
Type III collagen is vital for the formation of normal type I collagen fibrils in the cardiovascular system, intestines, and skin. In the extracellular matrix (ECM), type III collagen is a major component of the interstitial matrix. It is secreted by fibroblasts and other mesenchymal cell types, playing roles in various inflammation-associated pathologies such as lung injury, viral and nonviral liver diseases, kidney fibrosis, hernia, and vascular disorders
We introduce a type III collagen isolation and analysis protocol using a modified PAGE system with urea, which separates type I and III collagen in a gel. This method is advantageous because it is easy to perform and applicable with small amounts of samples. Read More
Native Collagen Detection by Type II Collagen ELISA
The results confirm that the kit can specifically detect native collagen. While the denaturing methods and pepsin digestion conditions may need optimization, the provided data is sufficient to understand the kit's functionality in detecting native collagen. Read More
Understanding the Hook Effect in a One-Step Sandwich ELISA
The Enzyme-Linked Immunosorbent Assay (ELISA) is a popular method used to detect and quantify soluble analytes such as proteins, hormones, and antibodies (1, 2). Despite its widespread application and high sensitivity, one notable limitation is the Hook Effect in a one-step assay protocol, a phenomenon that can lead to significantly inaccurate results in assays, especially at very high concentrations of... Read More
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