Native Collagen Detection by Type II Collagen ELISA
The results confirm that the kit can specifically detect native collagen. While the denaturing methods and pepsin digestion conditions may need optimization, the provided data is sufficient to understand the kit's functionality in detecting native collagen. Read More
Understanding the Hook Effect in a One-Step Sandwich ELISA
The Enzyme-Linked Immunosorbent Assay (ELISA) is a popular method used to detect and quantify soluble analytes such as proteins, hormones, and antibodies (1, 2). Despite its widespread application and high sensitivity, one notable limitation is the Hook Effect in a one-step assay protocol, a phenomenon that can lead to significantly inaccurate results in assays, especially at very high concentrations of... Read More
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