Scientific Articles

Salmon Type II Collagen Detection by Type II Collagen ELISA (Cat# 6018)

Salmon Type II Collagen Detection by Type II Collagen ELISA (Cat# 6018)

IntroductionType II collagen is an important structural protein that is essential for maintaining the strength and flexibility of cartilage and other connective tissues where it is mainly found. Recently, un-denatured (native) type II collagen has been used as a dietary supplement to support joint health (1). Many companies use chicken cartilage as a raw material, but fish cartilage can also... Read More

Monomeric and polymeric collagen in immunological assays

Monomeric and polymeric collagen in immunological assays

The quality of standards for assays is crucial for obtaining consistent results throughout long-term studies and between different studies. Using different batches of these reagents can impact assay validation and reproducibility. Chondrex, Inc.'s ELISA grade collagen undergoes filtration steps to remove polymeric collagen, ensuring a high ratio of monomeric collagen that is ideal for use as standards in sandwich ELISAs as well as antigens in indirect ELISAs.
To obtain absolute values of collagen levels in samples, monomeric collagen (ELISA grade collagen) must be used as a standard in ELISAs. Read More

Type III collagen purification and evaluation using SDS-PAGE

Type III collagen purification and evaluation using SDS-PAGE

Type III collagen is vital for the formation of normal type I collagen fibrils in the cardiovascular system, intestines, and skin. In the extracellular matrix (ECM), type III collagen is a major component of the interstitial matrix. It is secreted by fibroblasts and other mesenchymal cell types, playing roles in various inflammation-associated pathologies such as lung injury, viral and nonviral liver diseases, kidney fibrosis, hernia, and vascular disorders

We introduce a type III collagen isolation and analysis protocol using a modified PAGE system with urea, which separates type I and III collagen in a gel. This method is advantageous because it is easy to perform and applicable with small amounts of samples. Read More

Native Collagen Detection by Type II Collagen ELISA

Native Collagen Detection by Type II Collagen ELISA

The results confirm that the kit can specifically detect native collagen. While the denaturing methods and pepsin digestion conditions may need optimization, the provided data is sufficient to understand the kit's functionality in detecting native collagen. Read More

Understanding the Hook Effect in a One-Step Sandwich ELISA

Understanding the Hook Effect in a One-Step Sandwich ELISA

The Enzyme-Linked Immunosorbent Assay (ELISA) is a popular method used to detect and quantify soluble analytes such as proteins, hormones, and antibodies (1, 2). Despite its widespread application and high sensitivity, one notable limitation is the Hook Effect in a one-step assay protocol, a phenomenon that can lead to significantly inaccurate results in assays, especially at very high concentrations of... Read More

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