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New Mouse IgE Antibodies against Peanut Allergens for In-vivo and In-vitro Studies

New Mouse IgE Antibodies against Peanut Allergens for In-vivo and In-vitro Studies

RBL-2H3 cell degranulation with CPE and mouse anti-CPE IgE monoclonal antibodies*

Mouse peanut allergy models are used to study the pathogenesis of the peanut allergy and to evaluate new treatments. The mouse models can be induced by administration of crude peanut extract (CPE) or each purified Ara allergen and evaluated for humoral immune responses such as serum anti-IgE and IgG antibodies against the allergen, T-cell mediated immune response associated cytokines levels, as well as body temperature and clinical signs of anaphylaxis. These factor changes observed in the disease models are useful for studying the efficacy of protective effects against the development of allergic reactions (17).

To evaluate humoral immune responses against CPE in mouse allergy models, Chondrex, Inc. provides CPE (Catalog #3069) and anti-CPE IgE monoclonal antibody: Clone 2G11G7 (Catalog #3070)  for in-vitro and in-vivo studies.

Please refer to Chondrex, Inc's website for more information.

*Note: RBL-2H3 cells were cultured in 0.1 ml of DMEM containing 15% FBS at 10^6 cells/well in a 96-well plate at 37 degrees C for 3 hours and then treated with anti-CPE IgE monoclonal antibodies, 6E10C12 (circle), 2G11G7 (triangle), 6B7B10 (square), and 5E7B12 (diamond) at 37 degrees C for 16 hours. After washing cells with PBS two times, 2 ?g/ml of CPE in Tyrode’s buffer were added at 200  ? l/well and incubated at 37 degrees C for 1 hour. 100 ?l of samples from each well were transferred to a 96-well plate and assayed for beta-hexosaminidase activity. The degranulation of RBL-2H3 cells was expressed as a ratio compared with 100% degranulation of the cells which received 1% Triton-X in Tyrode’s buffer.

References

  1. J. J. Dolence, Induction of Peanut Allergy Through Inhalation of Peanut in Mice. Methods Mol. Biol. 2223, 19–35 (2021).
  2. M. K. Selgrade, C. C. Bowman, G. S. Ladics, L. Privalle, S. A. Laessig, Safety assessment of biotechnology products for potential risk of food allergy: implications of new research. Toxicol. Sci. 110, 31–39 (2009).
  3. X. M. Li, D. Serebrisky, S. Y. Lee, C. K. Huang, L. Bardina, B. H. Schofield, J. S. Stanley, A. W. Burks, G. A. Bannon, H. A. Sampson, A murine model of peanut anaphylaxis: T- and B-cell responses to a major peanut allergen mimic human responses. J. Aller
  4. M.-J. Bae, H. S. Shin, E.-K. Kim, J. Kim, D.-H. Shon, Oral administration of chitin and chitosan prevents peanut-induced anaphylaxis in a murine food allergy model. Int. J. Biol. Macromol. 61, 164–168 (2013).
  5. M. Kulis, X. Chen, J. Lew, Q. Wang, O. P. Patel, Y. Zhuang, K. S. Murray, M. W. Duncan, H. S. Porterfield, A. W Burks, S. C. Dreskin, The 2S albumin allergens of Arachis hypogaea, Ara h 2 and Ara h 6, are the major elicitors of anaphylaxis and can effecti
  6. L. M. Chang, Y. Song, X.-M. Li, H. A. Sampson, M. Masilamani, Dietary Elimination of Soybean Components Enhances Allergic Immune Response to Peanuts in BALB/c Mice. Int. Arch. Allergy Immunol. 166, 304–310 (2015).
  7. C. Zhou, T. Ludmila, N. Sun, C. Wang, Q. Pu, K. Huang, H. Che, BALB/c mice can be used to evaluate allergenicity of different food protein extracts. Food Agric. Immunol. 27, 589–603 (2016).

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