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Mouse - Anti-Collagen Type I & II Antibody Assay

In CIA-susceptible mice, the serum antibody levels to the type II collagen used for immunization are remarkably high, and importantly, these antibodies cross-react to autologous type II collagen, due to the conserved amino acid sequences of type II collagen.  Therefore, it is important to determine antibody levels to the heterologous type II collagen used for immunization, but it is more important to determine antibody levels to autologous collagen to accurately correlate antibody levels with the development and severity of arthritis in the CIA model.  Chondrex, Inc. provides complete ELISA kits for assaying mouse IgG antibodies to various species and types of collagen.

Mouse Anti-Collagen (I & II) IgG Antibody Assay Kit

Collagen Coated Wells Chromagen Catalog # Price (USD)
Bovine I OPD 1012 373.00
Bovine I TMB 1012T 373.00
Bovine II OPD 2032 383.00
Bovine II TMB 2032T 383.00
Chick I OPD 1011 373.00
Chick I TMB 1011T 373.00
Chick II OPD 2031 383.00
Chick II TMB 2031T 383.00
Human I OPD 1015 410.00
Human I TMB 1015T 410.00
Human II OPD 2035 421.00
Human II TMB 2035T 421.00
Mouse I OPD 1016 373.00
Mouse I TMB 1016T 373.00
Mouse II OPD 2036 475.00
Mouse II TMB 2036T 475.00
Porcine I OPD 1013 373.00
Porcine I TMB 1013T 373.00
Porcine II OPD 2033 383.00
Porcine II TMB 2033T 383.00

Mouse Anti-Type I and Type II Collagen IgG Assay Kit Protocol
Mouse Anti-Type I and Type II Collagen IgG Assay Kit with TMB Protocol
 

Cross-reactivity of Anti-Heterologous Type II Collagen Antibodies to Autologous Type II Collagen in Mouse CIA

This ELISA Kit is designed to determine anti-collagen antibodies in mouse collagen-induced arthritis model (CIA).  CIA is induced in mice, rats and non-human primates by immunizing with a heterolgous type II collagen rather than homologous collagen.  In CIA-susceptible mice, the serum antibody levels to the type II collagen used for immunization are remarkably high, and importantly, these antibodies cross-react to various species of type II collagen including autologous type II collagen by 50-60%, due to the conserved amino acid sequences of type II collagen.  Therefore, it is important to determine antibody levels to a heterologous type II collagen used for immunization, but it is more important to determine antibody levels cross-reacting to autologous collagen to correlate antibody levels to development and severity of arthritis in CIA model. Although type I collagen shares 80% of its amino acid sequence with type II collagen, it is not capable of inducing autoimmune-mediated diseases, which indicates that B-cell and T-cell epitope specificity is important for establishing autoimmunity and subsequent development of autoimmune diseases.  Therefore, type I collagen might be useful as a control for studying B-cell and T-cell epitope specificity.

Complete ELISA kits to measure mouse serum antibody levels to various species and types of collagen are available.  A standard mouse kit with one species of collagen will measure 39 samples.  Each kit contains a reference standard and reagents to perform two partial assays.  Customized kits to measure antibody levels to various species of type I and type II collagen can be custom ordered.