Cyanogen bromide digested peptide (CB-peptide) fragments of type II collagen are available for studying B-cell and T-cell epitopes of type II collagen. These peptides are pepsin-free and ideal for studying epitope specificity of T-cells by proliferation assay. These peptides are renatured into triple-helical conformation by a step-wise cooling method*, and can be used as an antigen for analyzing epitiope specificity of antibodies by ELISA.
In order to accommodate the variety of collagen studies available, Chondrex, Inc. offers different grades of collagen such as immunization grade, cell culture grade, ELISA grade, T-cell assay grade, and collagen CB fragments. If you need help deciding which grade of collagen is right for your study, please see our Understanding Chondrex, Inc.'s Grades of Purified Collagen article or contact us. For more information distinguishing between Type I and Type II collagen, please see our Type I & Type II Collagen: Structure Influences Staining Patterns article or contact us.
*See Reference: Collagen Rel Res. 5: 469-480 (1985).
Collagen Solubilizing Buffer
Bovine CB-Peptides of Type II Collagen
Chick CB-Peptides of Type II Collagen
Human CB-Peptides of Type II Collagen
Mouse CB-Peptides of Type II Collagen
