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Hemoglobin Assay

Hemoglobin is a heme protein carried by erythrocytes and transports oxygen from the lungs to the rest of the body. Therefore low hemoglobin levels in erythrocytes or low erythrocyte numbers in blood deprives the body of oxygen. This condition is defined as anemia.  This oxygen deficit can cause dizziness or headaches, while severe or long-lasting anemia can damage organs in the body and even result in death.  Anemia is also a complication of various inflammatory diseases such as sepsis (1-3) and autoimmune diseases (4, 5), and has been observed in rodent disease models of inflammatory bowel disease (IBD) (5, 6), colitis (7), sepsis (8), and autoimmune hemolytic anemia  (9-12).

Since hemoglobin levels correlate to the oxygen carrying-capacity of blood, hemoglobin is a useful marker of anemia to evaluate disease severity and effectiveness of treatments.  A standard hemoglobin assay utilizes the toxic cyanmethemoglobin method, however Chondrex introduces a safe non-cyanide hemoglobin assay kit (13, 14).  This assay kit correlates with the cyanmethemoglobin method and can be used to evaluate anemia in our mouse sepsis model and mouse IBD model, as well as other disease models.  Chondrex also provides related products such as, an HMGB1 detection kit (Sepsis), Fluorescent-labeled Dextrans (IBD), a D-xylose assay kit,  and Mouse anti-bacterial antibody ELISAs (IBD).

Hemoglobin Assay kit

Product Catalog # Price (USD)
Hemoglobin Assay kit 6024 90.00


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9. M. B. Jordan, N. van Rooijen, S. Izui, J. Kappler, P. Marrack, Liposomal clodronate as a novel agent for treating autoimmune hemolytic anemia in a mouse model. Blood 101, 594-601 (2003).

10. L. Fossati-Jimacket al., High pathogenic potential of low-affinity autoantibodies in experimental autoimmune hemolytic anemia. J Exp Med 190, 1689-1696 (1999).

11. D. Meyeret al., FcgammaRIII (CD16)-deficient mice show IgG isotype-dependent protection to experimental autoimmune hemolytic anemia. Blood 92, 3997-4002 (1998).

12. D. A. Schirmeret al., Mouse models of IgG- and IgM-mediated hemolysis. Blood 109, 3099-3107 (2007).

13. O. W. van Assendelft, W. G. Zijlstra, Observations on the alkaline haematin/detergent complex proposed for measuring haemoglobin concentration. J Clin Chem Clin Biochem 27, 191-195 (1989).

14. V. B. Shah, B. S. Shah, G. V. Puranik, Evaluation of non cyanide methods for hemoglobin estimation. Indian J Pathol Microbiol 54, 764-768 (2011).