Sepsis is defined as the manifestation of the systemic inflammatory response syndrome (SIRS) in the presence of infection. Currently, sepsis is hypothesized to involve at least two contrasting phenomena: SIRS, followed by the compensatory anti-inflammatory response syndrome (CARS). However, these syndromes can occur simultaneously in temporary homeostasis known as the mixed anti-inflammatory response syndrome (MARS) (1). Disruption of this homeostasis initiates a cascade of cellular signaling events producing severe effects in distant organs, such as inflammation and vascular collapse, which can lead to gastrointestinal dysfunction, compromised gut barriers, and bacterial translocation (2).
Thus, to better understand the complicated pathogenesis of sepsis, it is important to utilize an ideal experimental animal model of sepsis which consistently translates relevant information to the human condition. Sepsis animal models currently in use are Toxemia models (Endotoxemia models) (3, 4), Nonsurgical models (5), Live bacteria models (6), Surgical models (6), Implantation models (6), and Cecal ligation and puncture models (3, 4, 6).
Toxemia models of sepsis induced by bacteria components are widely used since they are convenient and reproducible. Lipopolysaccharide (LPS), the major structural component of the outer membrane of Gram-negative bacteria, is commonly used since it can be isolated in a relatively pure form and its activity reliably measured. These features allow for easy standardization in experimental studies (7).
Lipopolysaccharide (LPS) and Biotinylated LPS
|Product||Quantity||Catalog #||Price (USD)|
|Biotinylated Lipopolysaccharide from E. coli O111:B4||0.1 mg, lyophilized||6108||59.00|
|Lipopolysaccharide from E. coli 0111:B4||0.5 mg/ml x 5 ml||9028||80.00|
Chondrex, Inc. provides LPS in solution form, exhibiting a median lethal dose of about 500 ug in Balb/c mice. For toxemia models, 250 ug (0.5 ml) /mouse by IP injection can be used. However, optimization is required since dosage depends on animal vendors, strains, genetic background, age and housing conditions. Chondrex, Inc. also provides Biotinylated LPS to facilitate studies on LPS–host interactions. A biotinylated LPS and a streptavidin conjugated probe (an enzyme or a fluorochrome) can be used for identifying LPS ligands in many applications, such as: enzyme immunoassay, western blot, flow cytometry, and fluorescence microscopy (8).
Chondrex, Inc. manufacturers a variety of reagents and assay kit to aid sepsis researchers. Please see the links below or contact us for more information.
HMGB1 Related Products
HMGB1 is a late stage lethal mediator of endotoxin toxicity, and plays crucial roles as a pro-inflammatory cytokine in a variety of acute and chronic inflammatory diseases. HMGB1 not only triggers the release of inflammatory cytokines such as TNF-alpha and IL-6, but also increases the permeability of cell monolayers in tissue culture and mucosal permeability in mice. In sepsis models, serum HMGB1 has been shown to be a valuable marker for evaluating the disease mechanism, as well as mortality (4, 9-11). Chondrex, Inc provides an HMGB1 Detection ELISA Kit and a variety of anti-HMGB1 antibodies for in vivo and in vitro assays.
Permeability Evaluation Solution
Increased cellular permeability was recently demonstrated in sepsis models, thus providing useful information on the complex mechanism of this syndrome (10, 11). To elucidate the pathogenesis of sepsis, Chondrex, Inc. provides FITC-Dextran to evaluate the permeability of semi-permeable membranes to macromolecules in vivo and in vitro.
Anti-Bacterial Antibodies and Assay kits
The presence of pre-existing anti-endotoxin antibodies has been shown to mediate survival in sepsis in both humans and mice (12-14). In addition, anti-LPS antibodies have been shown to protect mice against LPS lethality in toxemia models (15, 16). To investigate the contribution of anti-endotoxin antibodies in sepsis, Chondrex, Inc. provides anti-LPS and anti-E. coli monoclonal antibodies, as well as ELISAs for the detection of anti-LPS and anti-E. coli antibodies in mouse and human biological fluids.