Mouse - IgE & Anti-OVA Antibody Assay

Ovalbumin (OVA) is widely used as an antigen for studying allergic diseases in mice. Chondrex provides three types of mouse IgE and two types of IgG anti-OVA antibody assay kits. These IgE assay kits employ an identical IgE standard (Clone E-C1), which allows for easy determination of total IgE and OVA specific IgE ratios in individual samples.

In addition, rat anti-mouse IgE monoclonal antibodies (Clone 345-2 and 77-1) used in these kits react to both IgEa and IgEb allotypes. Therefore, separate assays for IgEa and IgEb allotypes are not required.

Mouse IgE and Anti-OVA Antibody Assay Kits

Mouse Anti-OVA IgE Antibody Assay Kit

This kit is designed to simply determine anti-OVA IgE levels using an OVA-coated ELISA plate for samples used for    in vitro experiments, such as an IgE solution used for stimulating mast cells.  This ELISA kit includes reagents to measure 40 samples.  The assay range is approximately 0.8-50 ng/ml.

Mouse Serum Total IgE (IgE^a and IgE^b) Detection Kit

This kit is designed to determine serum total IgE (for IgE^a and IgE^b allotypes) levels.  The serum IgE level is often raised in allergic diseases and parasitic infections, although it does not reflect the allergic state and the clinical symptoms.  The Mouse Total IgE Detection Kit includes reagents to determine total IgE levels in 40 samples and the assay range is approximately 1.6-100 ng/ml.

Mouse Serum Anti-OVA IgE Antibody Assay Kit

This kit is designed to specifically detect IgE anti-OVA antibodies in mouse sera.  Mouse sera contain various types of antibodies, such as IgA, IgM, and IgG, against the same antigen at higher concentrations than IgE.  Therefore, it is difficult to detect IgE antibody levels due to competitive binding of these different types of antibodies on the same antigenic determinants.  This kit accurately measures anti-OVA IgE levels in samples of which the total IgE levels are less than 500 ng/ml.  This kit includes reagents for assaying 40 serum samples. The linear range of this assay is 0.4-25 ng/ml.

Mouse Anti-OVA IgG Assay Kit

The cross-linkage of IgE antibodies, which are bound to the receptor on mast cells by polyvalent allergen, triggers the degranulation of mast cells.  On the other hand, the pathogenic roles of IgG antibodies against the allergens are not well characterized.  The Mouse Anti-OVA IgG Assay Kit includes reagents to determine anti-OVA IgG antibody levels of 39 mouse serum samples and is used for studying the contributions of IgG in allergic diseases.  The linearity range of this assay is 0.2-12.5 ng/ml.

Mouse Anti-OVA IgG1 Antibody Assay Kit

This kit is designed to specifically detect IgG1 anti-OVA antibodies in mouse sera.  While the contribution of the interactions between IgE and its various cellular receptors has been well characterized, the role of IgG and more specifically IgG1 still needs more clarification.