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Anti-Ovalbumin Antibodies

Ovalbumin (OVA) is widely used as an antigen for studying the IgE-mediated allergic reaction in mice. Chondrex, Inc. provides mouse anti-OVA IgE, IgA, IgM and IgG monoclonal antibodies for the studies.

Allergenic IgE Monoclonal Antibodies

Product In Vitro Application In Vivo Application Quantity Catalog # Price (USD)
Mouse Anti OVA Monoclonal IgE Antibody E-C1 Hyper-Sensitivity Reaction 1 mg, lyophilized 3006 867.00
Mouse Anti OVA Monoclonal IgG1 Antibody L71 No 1 mg, lyophilized 3008 434.00
Mouse Anti-OVA Monoclonal IgE Antibody E-G5 No 1 mg, lyophilized 3007 434.00

Anti-Ovalbumin Antibodies

Product Quantity Catalog # Price (USD)
Mouse Anti-Ovalbumin IgA Monoclonal Antibody, Clone 2G12E12 1 mg/ml x 0.1 ml 7090 238.00
Mouse Anti-Ovalbumin IgE Monoclonal Antibody, Clone E-C1 1 mg/ml x 0.1 ml 7091 238.00
Mouse Anti-Ovalbumin IgE Monoclonal Antibody, Clone E-G5 1 mg/ml x 0.1 ml 7092 238.00
Mouse Anti-Ovalbumin IgG1 Monoclonal Antibody, Clone 2322 1 mg/ml x 0.1 ml 7094 238.00
Mouse Anti-Ovalbumin IgG1 Monoclonal Antibody, Clone L71 1 mg/ml x 0.1 ml 7093 238.00
Mouse Anti-Ovalbumin IgG2a Monoclonal Antibody, Clone M12E4D5 1 mg/ml x 0.1 ml 7095 238.00
Mouse Anti-Ovalbumin IgG2b Monoclonal antibody, Clone 4B4E6 1 mg/ml x 0.1 ml 7096 238.00
Mouse Anti-Ovalbumin IgG2c Monoclonal Antibody, Clone 3E3A9 1 mg/ml x 0.1 ml 7109 238.00
Mouse Anti-Ovalbumin IgM Monoclonal Antibody, Clone 2H11A8 1 mg/ml x 0.1 ml 7097 238.00
Mouse IgE Monoclonal Antibody, Isotype Control, Clone 2A101A12 1 mg/ml x 0.5 ml 7129 338.00

 

Allergenic and Non-Allergenic Antibodies
In general, the cross-linkage of two adjacent IgE antibody molecules, which are attached to IgE receptors on mast cells by a polyvalent allergen, is required to trigger degranulation (also known as activation) of mast cells (Figure 1a). Alternatively, IgG antibodies to an allergen can also trigger the degranulation of mast cells by forming cross-linkage of the allergen molecules captured by IgE antibodies on mast cells (Figure 1b).
 
Mast cell degranulation induced by cross-linkage of allergen specific IgE & IgG antibodies
 
Figure 1 - Two mechanisms of mast cell-bound IgE cross-linking. a) Cross-linkage by a single allergen and b) Cross-linkage by a single IgG antibody to two allergen molecules. Mouse IgE monoclonal antibody, Clone E-C1, is capable of inducing degranulation of mast cells in vitro (Figure 2a and 2b) and severe hypersensitivity in vivo (Figure 3), suggesting that clone E-C1 might recognize the repetitive epitopes of OVA. Furthermore, it has been reported that aggregated OVA carrying multiple epitopes increases the formation of cross-linkage of IgE molecules on mast cell surfaces. On the other hand, Clone E-G5 is not capable of inducing these allergic reactions in vitro or in vivo (Figures 1a, 1b, and 2), and can be used as a control.
 

Dose response & Clone comparison of Chondrex, Inc. Anti-OVA Allergenic Antibodies as measured by mast cell degranulation

Figure 2 - Degranulation of mast cells by monoclonal IgE antibody a) Comparison of E-C1 and E-G5 and b) Dose response of E-C1.

 

Comparison of mouse foot pad swelling induced by Chondrex, Inc. Anti-OVA Allergenic Antibodies and controls.

Figure 3 - Foot pad hypersensitivity reaction induced by E-C1 in Balb/c mice.