Mouse - Total Immunoglobulin Assay Kits

Total immunoglobulin isotype levels correlate to health or pathological conditions such as hypo- or hyper-gammaglobulinemia, and acute or chronic infections (1-5). In addition, total immunoglobulin levels are often analyzed relative to antigen-specific antibody levels to evaluate immune function in patients and animal disease models. Chondrex, Inc. provides mouse total immunoglobulin isotype ELISA kits for IgA, IgE, IgM, and IgG, as well as IgG subtypes: IgG1, IgG2a, IgG2b, and IgG3, along with anti-antigen antibody ELISA kits, such as autoantigens (ssDNA and dsDNA), dietary proteins (collagen and OVA), and bacteria (S. aureus, E. coli and LPS). 

In addition, these total immunoglobulin ELISA kits are also suitable for quantifying monoclonal antibody and polyclonal antibody isotype and/or subtype, as well as for establishing reliable antibody production monitoring and quality control methods.

Mouse Total Immunoglobulin Antibody Assay Kits

The gut barrier plays important roles in maintaining intestinal homeostasis (6-9). Oral immunization of ovalbumin (OVA) with cholera toxin-adjuvant enhances intestinal IgA against OVA. The immune response can be evaluated using the anti-OVA IgA antibody levels compared with total IgA levels. The following protocol may be useful for these studies.

Analysis of Antibodies in Intestinal Lavage

REFERENCES

1. K. J. Hamilton, M. Satoh, J. Swartz, H. B. Richards, W. H. Reeves, Influence of microbial stimulation on hypergammaglobulinemia and autoantibody production in pristane-induced lupus. Clin Immunol Immunopathol 86, 271-279 (1998).
2. C. B. Reimer et al., Hypergammaglobulinemia associated with human immunodeficiency virus infection. Monogr Allergy 23, 83-96 (1988).
3. S. Senda, E. Cheng, H. Kawanishi, IgG in murine intestinal secretions. Aging effect and possible physiological role. Scand J Immunol 29, 41-47 (1989).
4. Tana, S. Watarai, E. Isogai, K. Oguma, Induction of intestinal IgA and IgG antibodies preventing adhesion of verotoxin-producing Escherichia coli to Caco-2 cells by oral immunization with liposomes. Lett Appl Microbiol 36, 135-139 (2003).
5. M. C. Thurnheer, A. W. Zuercher, J. J. Cebra, N. A. Bos, B1 cells contribute to serum IgM, but not to intestinal IgA, production in gnotobiotic Ig allotype chimeric mice. J Immunol 170, 4564-4571 (2003).
6. Tana, S et al. Induction of intestinal IgA and IgG antibodies preventing adhesion of verotoxin-producing Escherichia coli to Caco-2 cells by oral immunization with liposomes. Lett Appl Microbiol 36, 135-139 (2003).
7. M. C. Thurnheer et al. B1 cells contribute to serum IgM, but not to intestinal IgA, production in gnotobiotic Ig allotype chimeric mice. J Immunol 170, 4564-4571 (2003).
8. S. Senda et al. IgG in murine intestinal secretions. Aging effect and possible physiological role. Scand J Immunol 29, 41-47 (1989).
9. N. Lycke et al. Lack of J chain inhibits the transport of gut IgA and abrogates the development of intestinal antitoxic protection. J Immunol 163, 913-919 (1999).