The semi-quantitative total tissue collagen detection kit (Sirius Red/Fast Green collagen staining kit: catalog # 9046) provides a simple quantitative micro-assay tool for determining the total amount of collagen and non-collagenous proteins in cultured cell layers and tissue sections by differential staining with two dyes, sirius red and fast green. Sirius Red binds to all types of collagen, whereas fast green stains non-collagenous proteins. This method has been applied to the measurement of collagen contents in various tissues.
Semi-Quantitative Tissue Total Collagen Staining Kit
|Product||Catalog #||Price (USD)|
|Sirius Red/Fast Green Collagen Staining Kit||9046||60.00|
These dyes can be easily extracted from stained tissues, and the amounts (micrograms) of collagen and non-collagenous proteins in each section can be calculated based on OD 540 (Sirius red) and OD 605 (fast green). The assay sensitivity is more than sufficient to determine collagen and non-collagenous proteins in 10-20 micrometer tissue sections prepared for general histological studies. The assay sensitivity for collagen is more than 3 micrograms/section, and non-collagenous protein is more than 50 microgram per section.