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Creatinine Assay Kit

Creatinine (2-Amino-1-methyl-2-imidazolin-4-one) is a product of creatine kinase activity in skeletal muscle.  Therefore serum creatinine levels are consistent depending on an individual’s muscle amount (1).  Serum creatinine is absorbed by the kidneys via glomerular filtration and then excreted.  Determining the glomerular filtration rate (GFR) using creatinine levels is a useful tool to evaluate renal function in renal diseases and impairments (2-4).  In addition, urinary creatinine levels are commonly used as an index of standardization for a variety of other tests (5-7). 

Chondrex, Inc provides a creatinine assay kit employing Jaffe Reaction (8).  The assay performs with only 30 µl of samples and a 30-minute assay time using a standard range of 400 - 6.3 µg/ml.  To standardize assay results between samples from human patients and animal models, this kit can be used together with urinary protein assays (Catalog # 6026 and 9040), albumin detection ELISA kits (Catalog # 3012 and 3020), NTX-I detection ELISA kit (Catalog # 6040), and CTX-I detection ELISA kit (Catalog # 6033) Please contact support@chondrex.com for more information.

Creatinine Assay Kit

Product Catalog # Price (USD)
Creatinine Assay Kit 6041 90.00

References

  1. S. B. Heymsfield, C. Arteaga, C. McManus, J. Smith, S. Moffitt, Measurement of muscle mass in humans: validity of the 24-hour urinary creatinine method. - PubMed - NCBI. The American Journal of Clinical Nutrition. 37, 478–494 (1983).
  2. J. A. Lustgarten, R. E. Wenk, Simple, rapid, kinetic method for serum creatinine measurement. Clinical Chemistry. 18, 1419–1422 (1972).
  3. S. R. Dunn, Z. Qi, E. P. Bottinger, M. D. Breyer, K. Sharma, Utility of endogenous creatinine clearance as a measure of renal function in mice. Kidney International. 65, 1959–1967 (2004).
  4. P. Fossati, L. Prencipe, G. Berti, Enzymic creatinine assay: a new colorimetric method based on hydrogen peroxide measurement. Clinical Chemistry. 29, 1494–1496 (1983).
  5. P. A. Peterson, P. E. Evrin, I. Berggård, Differentiation of glomerular, tubular, and normal proteinuria: determinations of urinary excretion of beta-2-macroglobulin, albumin, and total protein. J. Clin. Invest. 48, 1189–1198 (1969).
  6. M. J. Seibel, Biochemical markers of bone turnover: part I: biochemistry and variability. Clin Biochem Rev. 26, 97–122 (2005).
  7. S.-M. Ok et al., Concentrations of CTX I, CTX II, DPD, and PYD in the urine as a biomarker for the diagnosis of temporomandibular joint osteoarthritis: A preliminary study. Cranio. 38, 1–7 (2017).
  8. J. T. Clarke, Colorimetric Determination and Distribution of Urinary Creatinine and Creatine. Clinical Chemistry. 7, 271–283 (1961).