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Canine CRP Detection

Acute phase proteins (APPs) are blood proteins released from hepatocytes as an integral part of acute phase responses (APR).  The APR, as a part of the innate host defense system, is triggered by tissue damage and inflammation caused by infectious, immunologic, or neoplastic agents.  One of the well-characterized APPs, C-reactive protein (CRP) is an annular pentameric protein consisting of 224 amino acids (25kDa) (1).  CRP binds to the phosphocholine expressed on the surface of dead cells and bacteria (2).  These complexes activate the complement system, leading to the phagocytic removal of the complexes by macrophages.

CRP levels in canine serum are significantly elevated after inflammatory irritation, surgical trauma, or inflammatory diseases such as pyometra, panniculitis, acute pancreatitis, polyarthritis, septic arthritis, and hemangiosarcoma (3-7).  Therefore, serum CRP is considered a measure of inflammation in canines.  Interestingly, CRP is also found in canine saliva, and saliva CRP levels correlate with serum CRP levels.  Because blood collection is an invasive procedure, saliva collection presents an alternative method for CRP collection. Like serum CRP, saliva CRP can be used as a marker to monitor inflammation status (8). However, as saliva CRP levels are about 1% of serum CRP levels, a highly sensitive and reliable assay is required (5, 9-11).

Chondrex, Inc provides a canine CRP ELISA kit which is compatible with both serum and saliva samples. It can be used for detecting and monitoring canine inflammation, as well as in studies investigating inflammation.  An immunochromatographic test for veterinary use is currently in development.  Please contact Chondrex, Inc. at support@chondrex.com for more information.

Detection Assay

Product Catalog # Price (USD)
Canine CRP Detection Assay Kit 6027 347.00

References

  1. J. J. Ceron, P. D. Eckersall, S. Martynez-Subiela, Acute phase proteins in dogs and cats: current knowledge and future perspectives. Vet Clin Pathol 34, 85-99 (2005)
  2. S. Jain, V. Gautam, S. Naseem, Acute-phase proteins: As diagnostic tool. J Pharm Bioallied Sci 3, 118-127 (2011).
  3. J. G. Conner, P. D. Eckersall, J. Ferguson, T. A. Douglas, Acute phase response in the dog following surgical trauma. Res Vet Sci 45, 107-110 (1988).
  4. M. Nakamura , M. Takahashi, K. Ohno, A. Koshino, K. Nakashima, et al., C-reactive protein concentration in dogs with various diseases. J Vet Med Sci 70, 127-131 (2008).
  5. S. Yamamoto, S. Miyabi, N. Abe, K. Otabe, E. Furukawa, et al., Canine C-reactive protein (CRP) does not share common antigenicity with human CRP. Vet Res Commun 17, 259-266 (1993).
  6. M. Kjelgaard-Hansen, A. L. Jensen, G. A. Houser, L. R. Jessen, A. T. Kristensen, Use of serum C-reactive protein as an early marker of inflammatory activity in canine type II immune-mediated polyarthritis: case report. Acta Vet Scand 48, 9 (2006).
  7. A. Hillström, J. Bylin, R. Hagman, K. Björhall, H. Tvedten, et al., Measurement of serum C-reactive protein concentration for discriminating between suppurative arthritis and osteoarthritis in dogs. BMC Vet Res 12, 240 (2016).
  8. M. D. Parra, F. Tecles, S. Martinez-Subiela, J. J. Ceron, C-reactive protein measurement in canine saliva. J Vet Diagn Invest 17, 139-144 (2005).
  9. M. Kjelgaard-Hansen, A. L. Jensen, A. T. Kristensen, Evaluation of a commercially available human C-reactive protein (CRP) turbidometric immunoassay for determination of canine serum CRP concentration. Vet Clin Pathol 32, 81-87 (2003).
  10. M. Kjelgaard-Hansen, A. T. Kristensen, A. L. Jensen, Evaluation of a commercially available enzyme-linked immunosorbent assay (ELISA) for the determination of C-reactive protein in canine serum. J Vet Med A Physiol Pathol Clin Med 50, 164-168 (2003).
  11. A. Munoz-Prieto, A. Tvarijonaviciute, D. Escribano, S. Martinez-Subiela, J. J. Ceron, Use of heterologous immunoassays for quantification of serum proteins: The case of canine C-reactive protein. PLoS One 12, e0172188 (2017).