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Anti-Ro60 Antibodies

Sjögren’s syndrome (SS) is a chronic autoimmune rheumatic disease, which affects the lacrimal and saliva glands, resulting in dryness of the mouth and eyes (1).  This disease arises as a complication of lupus erythematosus, neonatal lupus syndrome, and rheumatoid arthritis (2).

Serum analysis in SS patients identified an autoantigen, Ro/SS-A 60kDa protein (Ro60), which is a RNA binding protein (3-5).  Anti-Ro/SSA antibodies are detected in 70 -100 % of SS patients and have been used for decades as a diagnostic marker for SS (6).  Furthermore, epitope analysis of Ro60 confirmed that several epitopes in the protein are highly antigenic, indicating that the specific antibodies against these amino acid sequences may be involved in developing the disease (7, 8).  More specifically, animal studies indicated that immunization with Ro60 peptides can elicit anti-Ro60 peptide antibodies in many mouse species, but only the Balb/c mouse strain will develop SS-like symptoms (9, 10).  Interestingly, naïve Balb/c mice developed SS from serum transfers of antiserum from Balb/c mice immunized with antigenic Ro60 peptides (11).  This suggests that not only are B-cell responses required for inducing SS, but that other species-relevant factors are involved as well.  Different IgG subclass levels were determined in these mouse strains, meaning that complement activating IgG subtypes such as IgG2a or IgG2b may play roles in the pathogenesis of the disease in a SS susceptible strain (12) .

Chondrex. Inc provides anti-Ro60 antibodies that recognize the 274-290 amino acid sequences of Ro60.  These monoclonal antibodies can be used to study the pathogenesis of the disease development in mice, especially in Balb/c mice. 

Mouse Anti-Ro60 Antibodies

Product Quantity Catalog # Price (USD)
Mouse Anti-Ro60 IgG1 Monoclonal Antibody, Clone 1G6B4 1 mg/ml x 0.1 ml 7124 175.00
Mouse Anti-Ro60 IgG2a Monoclonal Antibody, Clone 1G4H5 1 mg/ml x 0.1 ml 7126 175.00
Mouse Anti-Ro60 IgG2b Monoclonal Antibody, Clone 1B1E1 1 mg/ml x 0.1 ml 7125 175.00

References

1.        B. T. Kurien et al., Immunization with 60 kD Ro peptide produces different stages of preclinical autoimmunity in a Sjögren's syndrome model among multiple strains of inbred mice. Clin Exp Immunol. 173, 67–75 (2013).

2.        J. B. Harley, R. H. Scofield, Systemic lupus erythematosus: RNA-protein autoantigens, models of disease heterogeneity, and theories of etiology. J Clin Immunol. 11, 297–316 (1991).

3.        A. Halse, J. B. Harley, U. Kroneld, R. Jonsson, Ro/SS-A-reactive B lymphocytes in salivary glands and peripheral blood of patients with Sjögren's syndrome. Clin Exp Immunol. 115, 203–207 (1999).

4.        R. Yoshimi, A. Ueda, K. Ozato, Y. Ishigatsubo, Clinical and Pathological Roles of Ro/SSA Autoantibody System. Clinical and Developmental Immunology. 2012, 1–12 (2012).

5.        K. K. Gaither et al., Implications of anti-Ro/Sjögren's syndrome A antigen autoantibody in normal sera for autoimmunity. J. Clin. Invest. 79, 841–846 (1987).

6.        J. Wenzel et al., Antibodies targeting extractable nuclear antigens: historical development and current knowledge. Br. J. Dermatol. 145, 859–867 (2001).

7.        M. D. Rader, C. O'Brien, Y. S. Liu, J. B. Harley, M. Reichlin, Heterogeneity of the Ro/SSA antigen. Different molecular forms in lymphocytes and red blood cells. J. Clin. Invest. 83, 1293–1298 (1989).

8.        R. H. Scofield, A. D. Farris, A. C. Horsfall, J. B. Harley, Fine specificity of the autoimmune response to the Ro/SSA and La/SSB ribonucleoproteins. Arthritis & Rheumatism. 42, 199–209 (1999).

9.        N. Delaleu, H. Immervoll, J. Cornelius, R. Jonsson, Biomarker profiles in serum and saliva of experimental Sjögren's syndrome: associations with specific autoimmune manifestations. Arthritis Res. Ther. 10, R22–14 (2008).

10.      B. T. Kurien et al., Immunization with 60 kD Ro peptide produces different stages of preclinical autoimmunity in a Sjögren's syndrome model among multiple strains of inbred mice. Clin Exp Immunol. 173, 67–75 (2013).

11.      J. S. Maier-Moore et al., Passive transfer of antibodies to the linear epitope 60 kD Ro 273-289 induces features of Sjögren's syndrome in naive mice. Clin Exp Immunol. 180, 19–27 (2015).

12.      C. Nguyen et al., Role of complement and B lymphocytes in Sjögren's syndrome-like autoimmune exocrinopathy of NOD.B10-H2b mice. Molecular Immunology. 43, 1332–1339 (2006).