Sjögren’s syndrome (SS) is a chronic autoimmune rheumatic disease, which affects the lacrimal and saliva glands, resulting in dryness of the mouth and eyes (1). This disease arises as a complication of lupus erythematosus, neonatal lupus syndrome, and rheumatoid arthritis (2).
Serum analysis in SS patients identified an autoantigen, Ro/SS-A 60kDa protein (Ro60), which is a RNA binding protein (3-5). Anti-Ro/SSA antibodies are detected in 70 -100 % of SS patients and have been used for decades as a diagnostic marker for SS (6). Furthermore, epitope analysis of Ro60 confirmed that several epitopes in the protein are highly antigenic, indicating that the specific antibodies against these amino acid sequences may be involved in developing the disease (7, 8). More specifically, animal studies indicated that immunization with Ro60 peptides can elicit anti-Ro60 peptide antibodies in many mouse species, but only the Balb/c mouse strain will develop SS-like symptoms (9, 10). Interestingly, naïve Balb/c mice developed SS from serum transfers of antiserum from Balb/c mice immunized with antigenic Ro60 peptides (11). This suggests that not only are B-cell responses required for inducing SS, but that other species-relevant factors are involved as well. Different IgG subclass levels were determined in these mouse strains, meaning that complement activating IgG subtypes such as IgG2a or IgG2b may play roles in the pathogenesis of the disease in a SS susceptible strain (12) .
Chondrex. Inc provides anti-Ro60 antibodies that recognize the 273-289 amino acid sequences of Ro60. These monoclonal antibodies can be used to study the pathogenesis of the disease development in mice, especially in Balb/c mice.
Mouse Anti-Ro60 Antibodies
|Product||Quantity||Catalog #||Price (USD)|
|Mouse Anti-Ro60 IgG1 Monoclonal Antibody, Clone 1G6B4||1 mg/ml x 0.1 ml||7124||238.00|
|Mouse Anti-Ro60 IgG2a Monoclonal Antibody, Clone 1G4H5||1 mg/ml x 0.1 ml||7126||238.00|
|Mouse Anti-Ro60 IgG2b Monoclonal Antibody, Clone 1B1E1||1 mg/ml x 0.1 ml||7125||238.00|
1. B. T. Kurien et al., Immunization with 60 kD Ro peptide produces different stages of preclinical autoimmunity in a Sjögren's syndrome model among multiple strains of inbred mice. Clin Exp Immunol. 173, 67–75 (2013).
7. M. D. Rader, C. O'Brien, Y. S. Liu, J. B. Harley, M. Reichlin, Heterogeneity of the Ro/SSA antigen. Different molecular forms in lymphocytes and red blood cells. J. Clin. Invest. 83, 1293–1298 (1989).
9. N. Delaleu, H. Immervoll, J. Cornelius, R. Jonsson, Biomarker profiles in serum and saliva of experimental Sjögren's syndrome: associations with specific autoimmune manifestations. Arthritis Res. Ther. 10, R22–14 (2008).
10. B. T. Kurien et al., Immunization with 60 kD Ro peptide produces different stages of preclinical autoimmunity in a Sjögren's syndrome model among multiple strains of inbred mice. Clin Exp Immunol. 173, 67–75 (2013).