To effectively study the pathogenesis of nephritis caused by granular subepithelial deposition of IgG immune complexes, a well-characterized nephritis mouse model is required. A new nephritis model induced by immunization with a chemically modified cationic bovine serum albumin (cBSA) may provide an ideal tool for nephritis studies. Chondrex provides cBSA and offers an optimized immunization schedule for effectively inducing nephritis in Balb/c and ICR mice.
Mouse kidneys leak serum components such as bilirubin, resulting in overestimated urinary protein levels when assaying by the turbidity method, thus proteinuria is not a suitable marker for the evaluation of nephritis severity. On the other hand, albumin is the first protein observed in the urine when kidney dysfunction begins to develop, thus it is a more suitable marker for the evaluation of nephritis in mouse models. This kit employs a sandwich assay method, which requires only 4 hours to complete. In the mouse nephritis model, a mouse is deemed nephritic if the total albumin content in a 16-hour urine collection is greater than 1 mg. It is important to note that urinary albumin levels can reach up to 50-200 mg in a 16-hour urine collection, depending on the severity of nephritis.
Cationic Bovine Serum Albumin
|Product||Quantity||Catalog #||Price (USD)|
|Cationic Bovine Serum Albumin (cBSA)||20 mg (2mg/ml, 10 ml)||9058||83.00|
Mouse Urinary Albumin Detection Kit
|Product||Catalog #||Price (USD)|
|Mouse Albumin Detection Kit||3012||299.00|