Quick Menu

Collagen Detection

Collagen is the most abundant protein family that forms connective tissues and plays critical roles in the inflammatory process. In order to study the physiological and pathological turnover of connective tissues and for various research purposes, Chondrex provides unique kits and reagents for determining various species of type I and type II collagen. Each kit is designed to quantify the amount of native collagen in cultured tissues or tissue specimens by ELISA.  To aid researchers in deciding which kit to use, Chondrex provides a Collagen Detection Kit Selection Flowchart below.

Type I Collagen Detection Kit

Product Catalog # Price (USD)
Bovine Type I Collagen Detection Kit 6014 421.00
Canine Type I Collagen Detection Kit 6019 421.00
Human Type I Collagen Detection Kit 6021 469.00
Mouse Type I Collagen Detection Kit 6012 421.00
Porcine Type I Collagen Detection Kit 6015 421.00
Rabbit Type I Collagen Detection Kit 6016 421.00
Rat Type I Collagen Detection Kit 6013 421.00

Type II Collagen Detection Kit

Product Catalog # Price (USD)
Type II Collagen Detection Kit, Multi-Species 6018 421.00

Hydroxyproline Assay Kit

Product Catalog # Price (USD)
Hydroxyproline Assay Kit 6017 372.00

Proteinases For Collagen Solubilization

Product Catalog # Price (USD)
Elastase from Porcine Pancreas 30047 34.00
Pepsin 6011 9.00

Semi-Quantitative Tissue Total Collagen Detection Kit

Product Catalog # Price (USD)
Sirius Red/Fast Green Collagen Staining Kit 9046 55.00

Sirius Red Total Collagen Detection Kit

Product Catalog # Price (USD)
Sirius Red Total Collagen Detection Kit 9062 102.00

NOTE: Solubilization of collagen from samples is required for assaying collagen. Additional supplies such as guanidine hydrochloride, pepsin (catalog # 6011), elsatase (catalog # 30047) etc are necessary for processing samples.

Please contact customer service to obtain "Tips for Collagen Solubilization".

Brief Kit Descriptions

Type I Collagen Detection Kits

Type I collagen is a fibrilar collagen consisting of two identical alpha1(I) chains and one alpha2(I) chain. It is the most abundant collagen type and is found in most connective tissues such as skin, bone, tendon, ligament, and heart. Many tissues with the exception of bone, which consists of type I collagen, contain heterotypic fibrils with two or more distinct collagen types in coexistence. In general, type I collagen is dominant and type III collagen is a minor component. Chondrex provides 5 types of type I collagen assay kit for determining bovine, human, mouse, porcine and rat type I collagen.

Type II Collagen Detection Kit

Type II collagen is unique among the collagen family, and its tissue distribution is limited to avascular tissues such as cartilage and the vitreous body of the eyes. Since type II collagen can induce arthritis in experimental animals, autoimmunity to type II collagen is suspected in the pathogenesis of certain autoimmune diseases in humans such as rheumatoid arthritis, eye diseases associated with rheumatoid arthritis, and relapsing polychondritis, which affects specific tissues containing type II collagen. The Type II Collagen Detection Kit (catalog # 6018) is designed to quantify the amount of native type II collagen from various species (such as human, monkey, porcine, bovine, rat, mouse, rabbit, equine, and chick) in cultured tissues or tissue specimens by ELISA.

Semi-Quantitative Total Tissue Collagen Detection Kit (Sirius Red/Fast Green Collagen Staining Kit)

The semi-quantitative total tissue collagen detection kit (Sirius Red/Fast Green collagen staining kit: catalog # 9046) provides a simple quantitative micro-assay tool for determining the total amount of collagen and non-collagenous proteins in cultured cell layers and tissue sections by differential staining with two dyes, sirius red and fast green. Sirius Red binds to all types of collagen, whereas fast green stains non-collagenous proteins. This method has been applied to the measurement of collagen contents in various tissues.

These dyes can be easily extracted from stained tissues, and the amounts (micrograms) of collagen and non-collagenous proteins in each section can be calculated based on OD 540 (sirius red) and OD 605 (fast green). The assay sensitivity is more than sufficient to determine collagen and non-collagenous proteins in 10-20 micrometer tissue sections prepared for general histological studies. The assay sensitivity for collagen is more than 3 micrograms/section, and non-collagenous protein is more than 50 microgram per section.

Sirius Red Total Collagen Detection Kit 

Sirius red is a unique dye which specifically binds to the [Gly-X-Y]n helical structure on fibrillar collagen (type I - V). Sirius Red Total Collagen Detection Kit (Catalog # 9062) employs sirius red as a detection reagent determining collgen concentration in samples. However, this kit does not discriminate between collagen species and types.