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Mouse - Anti-Type II Collagen Subtype Antibody Assay

Autoantibodies to type II collagen play a primary role in inducing autoimmune-mediated inflammatory arthritis in the CIA model, but autoantibodies are not always capable of inducing arthritis. In mice, complement fixable IgG2a and IgG2b subtype autoantibody levels are pertinent in the induction of arthritis, as well as their epitope specificity. In order to adequately study the pathological roles of antibodies to type II collagen, Chondrex provides mouse anti-collagen IgG subtype (IgG1, IgG2a, IgG2b, IgG2c, and IgG3) antibody assay kits for further detailed analysis of antibodies in the mouse CIA model.

Mouse Anti-Type II Collagen IgG1 Subtype Antibody Assay Kit

Collagen Coated Wells Chromagen Catalog # Price (USD)
Bovine II OPD 20321 390.00
Bovine II TMB 20321T 390.00
Chick II OPD 20311 390.00
Chick II TMB 20311T 390.00
Human II OPD 20351 420.00
Human II TMB 20351T 420.00
Mouse II OPD 20361 470.00
Mouse II TMB 20361T 470.00
Porcine II OPD 20331 390.00
Porcine II TMB 20331T 390.00

Mouse Anti-Type II Collagen IgG2a Subtype Antibody Assay Kit

Collagen Coated Wells Chromagen Catalog # Price (USD)
Bovine II OPD 20322 390.00
Bovine II TMB 20322T 390.00
Chick II OPD 20312 390.00
Chick II TMB 20312T 390.00
Human II OPD 20352 420.00
Human II TMB 20352T 420.00
Mouse II OPD 20362 470.00
Mouse II TMB 20362T 470.00
Porcine II OPD 20332 390.00
Porcine II TMB 20332T 390.00

Mouse Anti-Type II Collagen IgG2b Subtype Antibody Assay Kit

Collagen Coated Wells Chromagen Catalog # Price (USD)
Bovine II OPD 20323 390.00
Bovine II TMB 20323T 390.00
Chick II OPD 20313 390.00
Chick II TMB 20313T 390.00
Human II OPD 20353 420.00
Human II TMB 20353T 420.00
Mouse II OPD 20363 470.00
Mouse II TMB 20363T 470.00
Porcine II OPD 20333 390.00
Porcine II TMB 20333T 390.00

Mouse Anti-Type II Collagen IgG2c Subtype Antibody Assay Kit

Collagen Coated Wells Chromagen Catalog # Price (USD)
Bovine II OPD 20325 390.00
Bovine II TMB 20325T 390.00
Chick II OPD 20315 390.00
Chick II TMB 20315T 390.00
Human II OPD 20355 420.00
Human II TMB 20355T 420.00
Mouse II OPD 20365 470.00
Mouse II TMB 20365T 470.00
Porcine II OPD 20335 390.00
Porcine II TMB 20335T 390.00

Mouse Anti-Type II Collagen IgG3 Subtype Antibody Assay Kit

Collagen Coated Wells Chromagen Catalog # Price (USD)
Bovine II OPD 20324 390.00
Bovine II TMB 20324T 390.00
Chick II OPD 20314 390.00
Chick II TMB 20314T 390.00
Human II OPD 20354 420.00
Human II TMB 20354T 420.00
Mouse II OPD 20364 470.00
Mouse II TMB 20364T 470.00
Porcine II OPD 20334 390.00
Porcine II TMB 20334T 390.00

Mouse Anti-Type II Collagen IgG Subtype Assay Kit Protocol
Mouse Anti-Type II Collagen IgG Subtype Assay Kit with TMB Protocol
 

In order to adequately study the pathological roles of antibodies to type II collagen in mouse CIA model, it is important to determine individual IgG subtype autoantibody levels to mouse type II collagen as well as the antibody levels to the heterologous type II collagen used for immunization.

A standard mouse kit with one species of collagen will measure 39 samples.  Each kit contains a reference standard and reagents to perform two partial assays.  Customized kits to measure antibody levels to various species of type I and type II collagen can be custom ordered.