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ChonBlock Western Blot Buffers

August 2019 Sale: 15% off all ChonBlock blocking buffers for ELISAs and Western Blots. Mention discount code AUG2019 when placing the order to receive the discount.

Traditional western blot buffers (BSA, Casein, NGS) provide an economical method for increasing the signal to noise ratio in a western blot. Many proprietary western blot buffers are more costly than these methods, but provide substantial blocking effects. ChonBlock™ is a new western blot buffer that proves to be as efficacious as other proprietary buffers, but at a cost-effective price.

ChonBlock Western Blot Buffers

Product Catalog # Price (USD)
ChonBlock Western Blot Buffer 1 (PBS) 9069P 99.00
ChonBlock Western Blot Buffer 1 (TBS) 9069T 99.00
ChonBlock Western Blot Buffer 2 for Goat (PBS) 90691P 49.00
ChonBlock Western Blot Buffer 2 for Goat (TBS) 90691T 49.00
ChonBlock Western Blot Buffer 2 for Rabbit (PBS) 90692P 49.00
ChonBlock Western Blot Buffer 2 for Rabbit (TBS) 90692T 49.00

A western blot was run using a 15% poly-Acrylamide gel under reducing conditions to compare three different buffer systems. Mouse Anti HMGB1 monoclonal antibody (Clone 1-1.2BP1) was used as a primary antibody at 1 µg/ml, and goat anti-mouse IgG antibody conjugated with HRP was used as a secondary antibody at a 1/4000 dilution. A non-specific smear signal was observed in the BSA-Tween buffer due to the lack of blocking effect. On the other hand, a competitor’s buffer prevented non-specific smearing, but showed a weak signal with high background color. In contrast, a distinct HMGB1 band with low background was observed using ChonBlock™.