Nephritogenic Monoclonal Antibodies

Goodpasture’s syndrome is a severe renal disease associated with pulmonary hemorrhage mediated by autoantibodies against the glomerular basement membrane (GBM). Chondrex provides unique rat monoclonal antibodies against the non-collagenous domain (NC1) of the alpha 4 chain of type IV collagen of GBM, which induces nephritis similar to Goodpasture’s syndrome in Wistar Kyoto (WKY) and the sub-strains of rats.

Product	Quantity	Catalog #	Price (USD)
Nephritogenic Monoclonal Antibody, Clone 114, 1 mg	1 mg/ml x 1 ml	70201	670.00
Nephritogenic Monoclonal Antibody, Clone 114, 5 mg	1 mg/ml x 5 ml	70205	3296.00
Nephritogenic Monoclonal Antibody, Clone a84, 1 mg	1 mg/ml x 1 ml	70211	670.00
Nephritogenic Monoclonal Antibody, Clone a84, 5 mg	1 mg/ml x 5 ml	70215	3296.00
Nephritogenic Monoclonal Antibody, Clone b35, 1 mg	1 mg/ml x 1 ml	70221	670.00
Nephritogenic Monoclonal Antibody, Clone b35, 5 mg	1 mg/ml x 5 ml	70225	3296.00

For Further Information: Rat Nephritis Protocol

Individual monoclonal antibodies induce mild to severe nephritis in rats within 1-2 days by a
single IP or IV injection.
Monoclonal antibody, clone b35 (IgG2b), induces severe nephritis associated with hematuria and pulmonary hemorrhage.
Monoclonal antibody, clone a84 (IgG2a), induces severe nephritis associated with hematuria.
Monoclonal antibody, clone 114 (IgG1), induces mild nephritis at the same dose of b35 and a84.

Susceptible and Non-Susceptible Strains of Rats
For unknown reasons, the susceptibility to nephritis is very limited to WKY and its sub-strains.
In addition, the susceptibility varies significantly among these sub-strains and the breeders as
shown in the table.

For example, WKY/NCrlCrlj (Charles River Japan) develop severe nephritis by a single IP or IV injection of anti-alpha 4 (IV)NC1 monoclonal antibodies (Figure 1). On the other hand, SHR/Crl rats (Charles River USA) are also susceptible to anti-alpha 4 (IV)NC1 monoclonal antibody-induced nephritis, but the severity is much lower than observed in WKY/NCrlCrlj as shown in Figure 2.

Figure 1 - Dose effect of nephritogenic monoclonal antibody on a) pulmonary hemorrhage and b) proteinuria
in WKY/NCrlCrlj (Charles River Japan)

Figure 2 Nephritis in SHR/Crl rats (Charles River USA)
Nephritis induced by a single IP injection of b35, a84, and 114 (100 mg/rat) in SHR/Crl (Charles River USA) was
much milder than the nephritis in WKY/NCrlCrlj (Charles River Japan).

Figure 3 – Histological changes in the kidney after injection of nephritogenic monoclonal antibodies
Apparent histological changes can be observed in the kidneys after injection of our nephritogenic monoclonal
antibodies such as enlarged glomeruli with severe endocapillary hypercellularity and extracapillary changes such as
capsular adhesion and crescent formation.