Peptides (less than 10 kDa) generally have very low antigenicity due to poor recognition by antigen presentation cells (APCs). In order to increase antigenicity, peptides should be conjugated to large molecule carrier proteins such as the widely-used bovine serum albumin (BSA) or keyhole limpet protein. For anti-peptide antibody production, cationic carrier proteins are beneficial over typical carrier proteins for the following reasons: 1) cationic proteins can hold more peptides because they contain more structural primary amines which generally work as conjugation sites for peptides, and 2) positively charged cationic proteins can be selectively processed as a presented antigen because of their high affinity for the negatively charged cell surfaces of APCs. As carrier proteins with high antigenicity and peptide conjugation efficiency, cationized BSA (cBSA) (Catalog # 9070) and cationized ovalbumin (cOVA) (Catalog # 9071) were prepared by substituting negatively charged carboxyl groups with positively-charged primary amines.
|Product||Catalog #||Price (USD)|
|Cationic Bovine Serum Albumin (cBSA)||9070||199.00|
|Cationic Ovalbumin (cOVA)||9071||199.00|