An article posted in Reprints.org from Resano et al. demonstrated that Chondrex, Inc.’s house dust mite (HDM) extract can be used as an allergen in experimental allergy models using human nasal epithelial cells (HNEpC). In addition, the changes in cytokine and chemokine levels in these experiments were evaluated using Chondrex, Inc.’s cytokine assay kits.
Elovanoids, which are bioactive lipid mediators, have shown promising anti-inflammatory properties in cellular experimental allergy models. HDM is the most prevalent indoor allergen, and exposure is tied to allergic respiratory diseases, including allergic rhinitis. The effects of elovanoids in HNEpC challenged with HDM extracts from D. pteronyssinus or D. farinae were evaluated using a comprehensive test panel, which included cell toxicity, viability, release of inflammatory cytokines, cellular senescence, and the expression of adhesion molecules.
HNEpC were challenged with different stressors or HDM extracts for 30 minutes, after which they were then treated with elovanoids. Cytotoxicity, cell viability, and the expression of pro-inflammatory cytokines and chemokines in the cell supernatant were evaluated. The study concluded that the HDM challenge in the cellular experimental allergy model induced expression of several key cytokines and chemokines linked to respiratory diseases and the treatment with elovanoids reduced cell toxicity and pro-inflammatory cytokine levels while increasing cell viability and anti-inflammatory cytokine levels. Overall, elovanoids might constitute a therapeutic option in the prevention and treatment of allergies to HDM.
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1. Resano, A.; Barajas, M.; Bhattacharjee, S.; Aguado-Jiménez, R.; Rodríguez, D.; Palacios, R.; Bazán, N.G. Elovanoid 34 Reduces Proinflammatory Cytokines and Arrest the Expression of Genes That Drive Senescence, Autophagy, Unfolded Protein Response (UPR), Matrix Metalloproteinases (MMPs) and Inflammation in Human Nasal Cells Exposed to House Dust Mite (HDM): Therapeutic Potential in Allergy. Preprints (2021).
In order to elicit allergic asthma models Chondrex, Inc. provides HDM extracts from D. pteronyssinus and D. farinae. To evaluate markers of allergic reaction and inflammation in the HDM allergic response, Chondrex, Inc. provides ELISA kits to analyze the levels of cytokines, chemokines, HDM, and anti-HDM antibodies in samples.
Please refer to Chondrex, Inc.’s website for more information.