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Anti-HMGB1 Antibodies

HMGB1 (high mobility group box 1 protein), also known as amphoterin, was recently identified as a late stage lethal mediator of endotoxin, and considered as a pro-inflammatory cytokine. Chondrex provides several monoclonal and polyclonal antibodies against synthetic peptides of HMGB1 and intact bovine HMGB1. These antibodies can be used for detecting HMGB1 by ELISA and western blot, or can be used to study the biological effects of HMGB1 in experiments in vitro and in vivo.

Anti-HMGB1 Monoclonal Antibodies

Product Cross-Reactivity Subtype Quantity Application Catalog # Price (USD)
Anti-Bovine HMGB1 Antibody, Clone 1-37.10FH B
H
Ms
R
Mouse IgG2a 1 mg/ml x 0.1 ml ELISA
WB
7047 175.00
Anti-Bovine HMGB1 Antibody, Clone 1-37.10FH, Biotinylated B
H
Ms
R
Mouse IgG2a 1 mg/ml x 0.1 ml ELISA
WB
70471 175.00
Anti-HMGB1 Peptide (166-176) Antibody, Clone 1-1.2.11.P2 B
H
Ms
R
Mouse IgG1 1 mg/ml x 0.1 ml ELISA
WB
7037 175.00
Anti-HMGB1 Peptide (166-176) Antibody, Clone 1-1.2.11.P2, Biotinylated B
H
Ms
R
Mouse IgG1 1 mg/ml x 0.1 ml ELISA
WB
70371 175.00
Anti-HMGB1 Peptide (2-11) Antibody, Clone 1-1.2BP1 B
H
Ms
R
Mouse IgG1 1 mg/ml x 0.1 ml ELISA
WB
7032 175.00
Anti-HMGB1 Peptide (2-11) Antibody, Clone 1-1.2BP1, Biotinylated B
H
Ms
R
Mouse IgG1 1 mg/ml x 0.1 ml ELISA
WB
70321 175.00
Anti-HMGB1 Peptide (2-11) Antibody, Clone 1-2.5AP1 B
H
Ms
R
Mouse IgG1 1 mg/ml x 0.1 ml ELISA
WB
7033 175.00
Anti-HMGB1 Peptide (2-11) Antibody, Clone 1-2.5AP1, Biotinylated B
H
Ms
R
Mouse IgG1 1 mg/ml x 0.1 ml ELISA
WB
70331 175.00
Anti-HMGB1 Peptide (2-11) Antibody, Clone 1-3.10EP1 B
H
Ms
R
Mouse IgG1 1 mg/ml x 0.1 ml ELISA
WB
7034 175.00
Anti-HMGB1 Peptide (2-11) Antibody, Clone 1-3.10EP1, Biotinylated B
H
Ms
R
Mouse IgG1 1 mg/ml x 0.1 ml ELISA
WB
70341 175.00

Anti-HMGB1 Polyclonal Antibodies

Product Cross-Reactivity Subtype Quantity Application Catalog # Price (USD)
Anti-Bovine HMGB1 IgG Antibodies B
H
Ms
R
Rabbit IgG 1 mg/ml x 0.1 ml ELISA
WB
7028 175.00
Anti-Bovine HMGB1 IgY Antibodies B
H
Ms
R
Chick IgY 1 mg/ml x 0.1 ml ELISA
WB
7064 175.00
Anti-HMGB1 Peptide (166-176) Antibodies B
H
Ms
R
Rabbit IgG 1 mg/ml x 0.1 ml ELISA
WB
7030 175.00
Anti-HMGB1 Peptide (2-11) Antibodies B
H
Ms
R
Rabbit IgG 1 mg/ml x 0.1 ml ELISA
WB
7029 175.00

HMGB1

HMGB1 is released by various types of cells such as macrophages, monocytes, and pituicytes exposed to LPS, and is considered a pro-inflammatory cytokine, which stimulates the release of other cytokines such as TNF-alpha and IL-6 through Toll-like receptor 4 (TLR4).  HMGB1 is considered a lethal mediator in sepsis, but may play important roles in chronic inflammatory diseases.

Specificity of Anti-HMGB1 Antibodies

The reactivity of individual antibodies with native and denatured HMGB1 was tested by ELISA.  Since the antibodies react equally with native and denatured HMGB1, species and type specificity of individual antibodies was tested by immunoblot analysis on 12% SDS-PAGE using partially purified HMGB1 and HMGB2 fractions from bovine and porcine thymus, in addition to cell lysates from rat C6, mouse RAW264.7, and human K562 cell lines.

All antibodies listed in the table cross-react to all species of HMGB1.  Some polyclonal and monoclonal anti-HMGB1 antibodies (catalog # 7032 and 7033) apparently cross-react with HMGB2 due to the high homology of amino acid sequences between HMGB1 and HMGB2