The type I hypersensitivity reaction mediated by IgE is a typical clinical feature of allergic diseases, such as asthma, eczema, hay fever, and urticaria. Ovalbumin (OVA) is widely used as an antigen for studying the IgE-mediated allergic reaction in mice. Chondrex provides allergenic and non-allergenic mouse anti-OVA IgE and IgG monoclonal antibodies for studying allergic diseases, such as in vivo hyper-sensitivity reactions, and related kits for in vitro and in vivo experiments.
Mouse Anti-OVA IgE and IgG Monoclonal Antibodies
|Product||In Vitro Application||In Vivo Application||Quantity||Catalog #||Price (USD)|
|Mouse Anti OVA Monoclonal IgG1 Antibody L71||No||1 mg, lyophilized||3008||406.00|
|Mouse Anti OVA Monoclonal IgE Antibody E-C1||Mast Cell Activation||Hyper-Sensitivity Reaction||1 mg, lyophilized||3006||812.00|
|Mouse Anti OVA Monoclonal IgE Antibody E-G5||No||No||1 mg, lyophilized||3007||406.00|
Allergenic and Non-Allergenic Antibodies
In general, the cross-linkage of two adjacent IgE antibody molecules, which are attached to IgE receptors on mast cells by a polyvalent allergen, is required to trigger degranulation (also known as activation) of mast cells (Figure 1a). Alternatively, IgG antibodies to an allergen can also trigger the degranulation of mast cells by forming cross-linkage of the allergen molecules captured by IgE antibodies on mast cells (Figure 1b).
Figure 1 - Two mechanisms of mast cell-bound IgE cross-linking. a) Cross-linkage by a single allergen and b) Cross-linkage by a single IgG antibody to two allergen molecules. A mouse IgE monoclonal antibody, Clone E-C1, is capable of inducing degranulation of mast cells in vitro (Figure 2a and 2b) and severe hypersensitivity in vivo (Figure 3), suggesting that clone E-C1 might recognize the repetitive epitopes of OVA. Furthermore, it has been reported that aggregated OVA carrying multiple epitopes increases the formation of cross-linkage of IgE molecules on mast cell surfaces. On the other hand, Clone E-G5 is not capable of inducing these allergic reactions in vitro or in vivo (Figures 1a, 1b, and 2), and can be used as a control.
Figure 2 - Degranulation of mast cells by monoclonal IgE antibodya) Comparison of E-C1 and E-G5 and b) Dose response of E-C1
Figure 3 - Foot pad hypersensitivity reaction induced by E-C1 in Balb/c mice