Collagen-Induced Arthritis (CIA) can be induced in certain strains of mice, rats, and non-human primates by immunization with type II collagen emulsified with adjuvant. To effectively induce CIA in animals, the type of adjuvant used for emulsifying collagen is a key element. Chondrex, Inc. provides both Incomplete Freund’s Adjuvant (IFA) and Complete Freund’s Adjuvant (CFA) containing various amounts of M. tuberculosis to optimize the immunization condition for inducing CIA.
**Please consult with your institution's animal care and use committee before ordering**
Note: For inducing CIA in rats, please use Incomplete Freund's Adjuvant for the emulsion preparation. Rats are susceptible to adjuvant-induced arthritis (AIA) when immunized with CFA, which leads to the development of a histologically and immunologically distinct type of arthritis than the CIA model.
Chondrex, Inc. provides various species and types of highly purified cartilage type II collagen, which are suitable for the induction of Collagen-Induced Arthritis (CIA). CIA can be induced in rodents and non-human primates by immunization with type II collagen. Choose the appropriate species and types of collagen for individual experimental purposes.
Incomplete and Complete Freund's Adjuvant for CIA
|Product||Content of M. Tuberculosis H-37 RA||Quantity||Catalog #||Price (USD)|
|Complete Freund's Adjuvant, 1 mg/ml||1 mg/ml||5 ml||7008||31.00|
|Complete Freund's Adjuvant, 2 mg/ml||2 mg/ml||5 ml||7009||36.00|
|Complete Freund's Adjuvant, 3 mg/ml||3 mg/ml||5 ml||7015||41.00|
|Complete Freund's Adjuvant, 4 mg/ml||4 mg/ml||5 ml||7001||54.00|
|Complete Freund's Adjuvant, 5 mg/ml||5 mg/ml||5 ml||7023||73.00|
|Incomplete Freund's Adjuvant||-----------||5 ml||7002||28.00|
Muramyl Dipeptide, MDP
|Product||Catalog #||Price (USD)|
|Muramyl Dipeptide, MDP, 2 mg||9065||74.00|
Type II Collagen
|Species||Quantity||Catalog #||Price (USD)|
|Bovine||10 mg, lyophilized||20021||195.00|
|Bovine||2 mg/ml x 5 ml||20022||195.00|
|Chick||10 mg, lyophilized||20011||195.00|
|Chick||2 mg/ml x 5 ml||20012||195.00|
|Porcine||10 mg, lyophilized||20031||195.00|
|Porcine||2 mg/ml x 5 ml||20032||195.00|
The following information has to be considered for induction of arthritis.
Combination of MHC Types and Species of Collagen for Inducing CIA
Collagen-induced arthritis (CIA) can be induced in certain strains of mice, rats, and non-human primates by immunization with type II collagen emulsified with adjuvant. Susceptibility to CIA in rodents is linked to MHC class II molecules of animals, but also depends on the species of type II collagen used for immunization. Choose the appropriate species of type II collagen for inducing CIA according to the table below. Importantly, homologous type II collagen is not capable of inducing CIA by an ordinary immunization schedule.
Two Factors that should be considered by Users of Animal Models
Animal models have contributed to remarkable advances in the understanding of numerous human diseases. As invaluable as animal models are, some drawbacks do exist. One such drawback stems from the different sources of variability which can affect the outcome of the experiments. Therefore, a pilot study is generally recommended for first time users of all animal models. A brief discussion of the two main points of variability will be explained below.
1. Animal Vendors
The genetic background of mice, even in the same strain, will vary from vendor to vendor and can affect how the animals respond to various reagents, thus impacting the results of your experiment (1). Therefore, testing animals from different vendors using a defined protocol is recommended before proceeding with a full-scale experiment.
2. Housing Conditions
The bacteria flora is very important to the health of the animal, including a properly functioning immune system (1). Therefore, we recommend housing animals in Specific Pathogen Free (SPF) conditions rather than conventional conditions to avoid variability caused by bacteria.
Overall, special consideration of these main points of variability will aid the researcher in completing their experiments with less confounding results.
1. Ivanov II. et al. Induction of intestinal Th17 cells by segmented filamentous bacteria. Cell 139(3): 485-498 (2009).