Mouse Urinary Albumin Detection

Mouse kidneys leak serum components such as bilirubin, resulting in overestimated urinary protein levels when assaying by the turbidity method, thus proteinuria is not a suitable marker for the evaluation of nephritis severity. On the other hand, albumin is the first protein observed in the urine when kidney dysfunction begins to develop, thus it is a more suitable marker to evaluate the severity of nephritis in mouse models.

This kit employs a sandwich assay method, which requires only 4 hours to complete. In the mouse nephritis model, a mouse is deemed nephritic if the total albumin content in a 16-hour urine collection is greater than 1 mg. It is important to note that urinary albumin levels can reach up to 50-200 mg in a 16-hour urine collection, depending on the severity of nephritis.

Mouse Urinary Albumin Detection Kit