Allergenic IgE Monoclonal Antibodies

^C^hondrex

Chondrex provides two IgE monoclonal antibodies specific to ovalbumin (OVA), clone E-C1 and clone E-G6, in addition to one IgG1 monoclonal antibody against OVA, clone L71. In general, the cross-linkage of IgE molecules bound to the receptor on mast cells by a polyvalent allergen is required to trigger degranulation of mast cells. Alternatively, IgG antibody to the allergens can trigger the activation of mast cells by cross-linking two antigens bound by IgE antibodies on mast cell surfaces. However, E-C1 alone is capable of inducing degranulation of mast cells in vitro (Figure 1), and severe hypersensitivity reaction in vivo (Figure 2). Therefore, it is assumed that E-C1 might recognize repetitive epitopes of OVA. Futhermore, it has been reported that aggregated OVA carrying multiple epitopes, increases the formation of cross-linkage of IgE on mast cell surfaces. On the other hand, E-G5 is not capable of inducing these hypersensitivity reactions by itself in vitro and in vivo (Figures 1 and 2), and can be used as a control.

Figure 1 - Degranulation of mast cells by monoclonal IgE antibody
1A - Comparison of three monoclonal antibodies

1B - Dose response of EC-1 and E-G5

Figure 2 - Foot pad hypersensitivity reaction induced by EC-1 in Balb/c mice

Catalog #	Description	Price (USD)
3006	Mouse monoclonal IgE antibody E-C1, 1 mg	$750
3007	Mouse monoclonal IgE antibody E-G5, 1 mg	$375
3008	Mouse monoclonal IgG1 antibody L71, 1 mg	$375

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